Electroactive nanoinjection platform for intracellular delivery and gene silencing | Journal of Nanobiotechnology
Stewart MP, Langer R, Jensen KF. Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts. Chem Rev. 2018;118:7409–531.
Tay A, Melosh N. Nanostructured materials for intracellular cargo delivery. Acc Chem Res. 2019;52:2462–71.
Morshedi Rad D, Alsadat Rad M, Razavi Bazaz S, Kashaninejad N, Jin D. Ebrahimi Warkiani M. A Comprehensive Review on Intracellular Delivery. Adv Mater. 2021;33:e2005363.
Yoh H, Aslanoglou S, Lestrell E, Shokouhi A-R, Belcher S, Thissen H, Voelcker NH, Elnathan R. Chapter TEN – Cellular nanotechnologies: Orchestrating cellular processes by engineering silicon nanowires architectures. In: Semiconducting Silicon Nanowires for Biomedical Applications (Second Edition) Edited by Coffer J: Woodhead Publishing; 2022: 231–278.
Venugopalan PL, Esteban-Fernández de Ávila B, Pal M, Ghosh A, Wang J. Fantastic voyage of nanomotors into the cell. ACS Nano. 2020;14:9423–39.
Abbott J, Ye T, Ham D, Park H. Optimizing nanoelectrode arrays for scalable intracellular electrophysiology. Acc Chem Res. 2018;51:600–8.
Spira ME, Hai A. Multi-electrode array technologies for neuroscience and cardiology. Nat Nanotechnol. 2013;8:83–94.
Pei F, Tian B. Nanoelectronics for minimally invasive Cellular Recordings. Adv Funct Mater. 2020;30:1906210.
Dipalo M, Caprettini V, Bruno G, Caliendo F, Garma LD, Melle G, Dukhinova M, Siciliano V, Santoro F, De Angelis F. Membrane poration mechanisms at the cell–nanostructure interface. Adv Biosyst. 2019;3:1900148.
Fesnak AD, June CH, Levine BL. Engineered T cells: the promise and challenges of cancer immunotherapy. Nat Rev Cancer. 2016;16:566–81.
DiTommaso T, Cole JM, Cassereau L, Buggé JA, Hanson JLS, Bridgen DT, Stokes BD, Loughhead SM, Beutel BA, Gilbert JB, Nussbaum K, Sorrentino A, Toggweiler J, Schmidt T, Gyuelveszi G, Bernstein H, Sharei A. Cell engineering with microfluidic squeezing preserves functionality of primary immune cells in vivo. Proc Natl Acad Sci. 2018;115:E10907–14.
Soltani Dehnavi S, Eivazi Zadeh Z, Harvey AR, Voelcker NH, Parish CL, Williams RJ, Elnathan R, Nisbet DR. Changing fate: reprogramming cells via Engineered Nanoscale Delivery materials. Adv Mater. 2022;34:2108757.
Stewart MP, Sharei A, Ding X, Sahay G, Langer R, Jensen KF. In vitro and ex vivo strategies for intracellular delivery. Nature. 2016;538:183–92.
Higgins SG, Becce M, Belessiotis-Richards A, Seong H, Sero JE, Stevens MM. High-aspect-ratio Nanostructured Surfaces as Biological Metamaterials. Adv Mater. 2020;32:e1903862.
Elnathan R, Barbato MG, Guo X, Mariano A, Wang Z, Santoro F, Shi P, Voelcker NH, Xie X, Young JL, Zhao Y, Zhao W, Chiappini C. Biointerface design for vertical nanoprobes. Nat Rev Mater. 2022;7:953–73.
Chen Y, Alba M, Tieu T, Tong Z, Minhas RS, Rudd D, Voelcker NH, Cifuentes-Rius A, Elnathan R. Engineering Micro–Nanomaterials for Biomedical Translation. Adv NanoBiomed Res. 2021;1:2100002.
Wilbie D, Walther J, Mastrobattista E. Delivery aspects of CRISPR/Cas for in vivo genome editing. Acc Chem Res. 2019;52:1555–64.
Doudna JA, Charpentier E. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346:1258096.
Chen Y, Aslanoglou S, Murayama T, Gervinskas G, Fitzgerald LI, Sriram S, Tian J, Johnston APR, Morikawa Y, Suu K, Elnathan R, Voelcker NH. Silicon-Nanotube-Mediated intracellular delivery enables Ex vivo gene editing. Adv Mater. 2020;32:e2000036.
Rabinovich PM, Komarovskaya ME, Ye Z-J, Imai C, Campana D, Bahceci E, Weissman SM. Synthetic messenger RNA as a tool for gene therapy. Hum Gene Ther. 2006;17:1027–35.
Gopal S, Chiappini C, Penders J, Leonardo V, Seong H, Rothery S, Korchev Y, Shevchuk A, Stevens MM. Porous silicon nanoneedles modulate endocytosis to deliver biological payloads. Adv Mater. 2019;31:1806788.
Kinoshita M, Hynynen K. A novel method for the intracellular delivery of siRNA using microbubble-enhanced focused ultrasound. Biochem Biophys Res Commun. 2005;335:393–9.
Chen Y, Aslanoglou S, Gervinskas G, Abdelmaksoud H, Voelcker NH, Elnathan R. Cellular Deformations Induced by Conical Silicon Nanowire arrays facilitate Gene Delivery. Small. 2019;15:e1904819.
Kim D, Kim C-H, Moon J-I, Chung Y-G, Chang M-Y, Han B-S, Ko S, Yang E, Cha KY, Lanza R, Kim K-S. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. 2009;4:472.
Shalek AK, Robinson JT, Karp ES, Lee JS, Ahn D-R, Yoon M-H, Sutton A, Jorgolli M, Gertner RS, Gujral TS, MacBeath G, Yang EG, Park H. Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells. Proc Natl Acad Sci. 2010;107:1870–5.
Bruce VJ, McNaughton BR. Inside job: methods for delivering proteins to the interior of mammalian cells. Cell Chem Biol. 2017;24:924–34.
Nitin N, LaConte L, Zurkiya O, Hu X, Bao G. Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent. J Biol Inorg Chem. 2004;9:706–12.
McDougall C, Stevenson DJ, Brown CT, Gunn-Moore F, Dholakia K. Targeted optical injection of gold nanoparticles into single mammalian cells. J Biophotonics. 2009;2:736–43.
Chiappini C, Martinez JO, De Rosa E, Almeida CS, Tasciotti E, Stevens MM. Biodegradable nanoneedles for localized delivery of nanoparticles in vivo: exploring the biointerface. ACS Nano. 2015;9:5500–9.
Huang JA, Caprettini V, Zhao Y, Melle G, Maccaferri N, Deleye L, Zambrana-Puyalto X, Ardini M, Tantussi F, Dipalo M, De Angelis F. On-Demand intracellular delivery of single particles in single cells by 3D hollow nanoelectrodes. Nano Lett. 2019;19:722–31.
Elnathan R, Kantaev R, Patolsky F. Synthesis of Hybrid Multicomponent Disklike Nanoparticles. Nano Lett. 2008;8:3964–72.
Chiappini C, De Rosa E, Martinez J, Liu X, Steele J, Stevens M, Tasciotti E. Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization. Nat Mater. 2015;14:532–9.
Huang D, Zhao D, Wang X, Li C, Yang T, Du L, Wei Z, Cheng Q, Cao H, Liang Z, Huang Y, Li Z. Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array. Theranostics. 2018;8:2361.
Soofiyani SR, Baradaran B, Lotfipour F, Kazemi T, Mohammadnejad L. Gene therapy, early promises, subsequent problems, and recent breakthroughs. Adv Pharm Bull. 2013;3:249.
Chiappini C, Chen Y, Aslanoglou S, Mariano A, Mollo V, Mu H, De Rosa E, He G, Tasciotti E, Xie X. Tutorial: using nanoneedles for intracellular delivery. Nat Protoc. 2021;16:4539–63.
Elnathan R, Tay A, Voelcker NH, Chiappini C. The start-ups taking nanoneedles into the clinic. Nat Nanotechnol. 2022;17:807–11.
Schmiderer L, Subramaniam A, Žemaitis K, Bäckström A, Yudovich D, Soboleva S, Galeev R, Prinz CN, Larsson J, Hjort M. Efficient and nontoxic biomolecule delivery to primary human hematopoietic stem cells using nanostraws. Proc Natl Acad Sci. 2020;117:21267–73.
Sadelain M, Rivière I, Riddell S. Therapeutic T cell engineering. Nature. 2017;545:423–31.
Tay A, Melosh N. Mechanical stimulation after centrifuge-free Nano‐Electroporative transfection is efficient and maintains long‐term T cell functionalities. Small. 2021;17:2103198.
Chen Y, Wang J, Li X, Hu N, Voelcker NH, Xie X, Elnathan R. Emerging roles of 1D vertical nanostructures in orchestrating immune cell functions. Adv Mater. 2020;32:2001668.
Kumar ARK, Shou Y, Chan B, Tay LK. Materials for improving Immune cell transfection. Adv Mater. 2021;33:e2007421.
Chen Y, Mach M, Shokouhi A-R, Yoh HZ, Bishop DC, Murayama T, Suu K, Morikawa Y, Barry SC, Micklethwaite K, Elnathan R, Voelcker NH. Efficient non-viral CAR-T cell generation via silicon-nanotube-mediated transfection. Mater Today. 2023;64:8–17.
Shokouhi A-R, Chen Y, Yoh HZ, Brenker J, Alan T, Murayama T, Suu K, Morikawa Y, Voelcker NH, Elnathan R. Engineering efficient Car-T cells via Electroactive Nanoinjection. Adv Mater n/a:2304122. https://doi.org/10.1002/adma.202304122.
Naldini L. Gene therapy returns to centre stage. Nature. 2015;526:351–60.
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS, Verfaillie CM. Efficient transfection of embryonic and adult stem cells. Stem Cells. 2004;22:531–43.
Lestrell E, Chen Y, Aslanoglou S, O’Brien CM, Elnathan R, Voelcker NH. Silicon Nanoneedle-Induced Nuclear deformation: implications for human somatic and stem cell nuclear mechanics. ACS Appl Mater Interfaces. 2022;14:45124–36.
Lestrell E, O’Brien CM, Elnathan R, Voelcker NH. Vertically aligned nanostructured topographies for human neural stem cell differentiation and neuronal cell interrogation. Adv Ther. 2021;4:2100061.
Lestrell E, Patolsky F, Voelcker NH, Elnathan R. Engineered nano-bio interfaces for intracellular delivery and sampling: applications, agency and artefacts. Mater Today. 2020;33:87–104.
Yang NJ, Hinner MJ. Getting across the cell membrane: an overview for small molecules, peptides, and proteins. Methods Mol Biol. 2015;1266:29–53.
Shi B, Xue M, Wang Y, Wang Y, Li D, Zhao X, Li X. An improved method for increasing the efficiency of gene transfection and transduction. Int J Physiol Pathophysiol Pharmacol. 2018;10:95.
Wang B, Ma R, Liu G, Li Y, Liu X, An Y, Shi L. Glucose-responsive micelles from self-assembly of poly (ethylene glycol)-b-poly (acrylic acid-co-acrylamidophenylboronic acid) and the controlled release of insulin. Langmuir. 2009;25:12522–8.
Thomas CE, Ehrhardt A, Kay MA. Progress and problems with the use of viral vectors for gene therapy. Nat Rev Genetics. 2003;4:346–58.
Kotterman MA, Chalberg TW, Schaffer DV. Viral vectors for gene therapy: translational and clinical outlook. Annu Rev Biomed Eng. 2015;17:63–89.
Dinca A, Chien W-M, Chin MT. Intracellular delivery of proteins with cell-penetrating peptides for therapeutic uses in human disease. Int J Mol Sci. 2016;17:263.
Kristensen M, Birch D, Mørck Nielsen H. Applications and challenges for use of cell-penetrating peptides as delivery vectors for peptide and protein cargos. Int J Mol Sci. 2016;17:185.
Fang J, Hsueh Y-Y, Soto J, Sun W, Wang J, Gu Z, Khademhosseini A, Li S. Engineering biomaterials with micro/nanotechnologies for cell reprogramming. ACS Nano. 2020;14:1296–318.
Sharei A, Poceviciute R, Jackson EL, Cho N, Mao S, Hartoularos GC, Jang DY, Jhunjhunwala S, Eyerman A, Schoettle T, Langer R, Jensena KF. Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform. Integr Biol. 2014;6:470–5.
Sharei A, Zoldan J, Adamo A, Sim WY, Cho N, Jackson E, Mao S, Schneider S, Han M-J, Lytton-Jean A, Basto PA, Jhunjhunwala S, Lee J, Heller DA, Kang JW, Hartoularos GC, Kim K-S, Anderson DG, Langer R, Jensen KF. A vector-free microfluidic platform for intracellular delivery. Proc Natl Acad Sci. 2013;110:2082–7.
Matsumoto D, Rao Sathuluri R, Kato Y, Silberberg YR, Kawamura R, Iwata F, Kobayashi T, Nakamura C. Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells. Sci Rep. 2015;5:15325.
Kang G, Carlson DW, Kang TH, Lee S, Haward SJ, Choi I, Shen AQ, Chung AJ. Intracellular nanomaterial delivery via spiral hydroporation. ACS Nano. 2020;14:3048–58.
Joo B, Hur J, Kim GB, Yun SG, Chung AJ. Highly efficient transfection of human primary T lymphocytes using droplet-enabled mechanoporation. ACS Nano. 2021;15:12888–98.
Wang T, Chen H, Yu C, Xie X. Rapid determination of the electroporation threshold for bacteria inactivation using a lab-on-a-chip platform. Environ Int. 2019;132:105040.
Boukany PE, Morss A, Liao W-c, Henslee B, Jung H, Zhang X, Yu B, Wang X, Wu Y, Li L. Nanochannel electroporation delivers precise amounts of biomolecules into living cells. Nat Nanotechnol. 2011;6:747–54.
Im DJ, Jeong S-N. Transfection of Jurkat T cells by droplet electroporation. Biochem Eng J. 2017;122:133–40.
Patino CA, Pathak N, Mukherjee P, Park SH, Bao G, Espinosa HD. Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering. Sci Adv. 2022;8:eabn7637.
Gupta P, Kar S, Kumar A, Tseng F-G, Pradhan S, Mahapatra PS, Santra TS. Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids. Analyst. 2021;146:4756–66.
Wu Y-C, Wu T-H, Clemens DL, Lee B-Y, Wen X, Horwitz MA, Teitell MA, Chiou P-Y. Massively parallel delivery of large cargo into mammalian cells with light pulses. Nat Methods. 2015;12:439–44.
Ramesan S, Rezk AR, Dekiwadia C, Cortez-Jugo C, Yeo LY. Acoustically-mediated intracellular delivery. Nanoscale. 2018;10:13165–78.
Yoon S, Kim MG, Chiu CT, Hwang JY, Kim HH, Wang Y, Shung KK. Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound. Sci Rep. 2016;6:20477.
Meacham JM, Durvasula K, Degertekin FL, Fedorov AG. Enhanced intracellular delivery via coordinated acoustically driven shear mechanoporation and electrophoretic insertion. Sci Rep. 2018;8:3727.
Qu Y, Zhang Y, Yu Q, Chen H. Surface-mediated intracellular delivery by physical membrane disruption. ACS Appl Mater Interfaces. 2020;12:31054–78.
Ding X, Stewart MP, Sharei A, Weaver JC, Langer RS, Jensen KF. High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption. Nat Biomed Eng. 2017;1:0039.
Aghaamoo M, Chen YH, Li X, Garg N, Jiang R, Yun JT, Lee AP. High-throughput and dosage-controlled intracellular delivery of large cargos by an acoustic-electric Micro-Vortices platform. Adv Sci. 2022;9:e2102021.
Hur J, Chung AJ. Microfluidic and nanofluidic intracellular delivery. Adv Sci. 2021;8:2004595.
Chang L, Li L, Shi J, Sheng Y, Lu W, Gallego-Perez D, Lee LJ. Micro-/nanoscale electroporation. Lab Chip. 2016;16:4047–62.
Shi J, Ma Y, Zhu J, Chen Y, Sun Y, Yao Y, Yang Z, Xie J. A review on electroporation-based intracellular delivery. Molecules. 2018;23:3044.
Balantic K, Miklavcic D, Krizaj I, Kramar P. The good and the bad of cell membrane electroporation. Acta Chim Slov. 2021;68:753–64.
Choi S-E, Khoo H, Hur SC. Recent advances in Microscale Electroporation. Chem Rev. 2022;122:11247–86.
Shokouhi A-R, Aslanoglou S, Nisbet D, Voelcker NH, Elnathan R. Vertically configured nanostructure-mediated electroporation: a promising route for intracellular regulations and interrogations. Mater Horiz. 2020;7:2810–31.
Tay A. The benefits of going small: nanostructures for mammalian cell transfection. ACS Nano. 2020;14:7714–21.
Yoh HZ, Chen Y, Aslanoglou S, Wong S, Trifunovic Z, Crawford S, Lestrell E, Priest C, Alba M, Thissen H, Voelcker NH, Elnathan R. Polymeric nanoneedle arrays mediate stiffness-independent intracellular delivery. Adv Funct Mater. 2022;32:2104828.
Li X, Matino L, Zhang W, Klausen L, McGuire AF, Lubrano C, Zhao W, Santoro F, Cui B. A nanostructure platform for live-cell manipulation of membrane curvature. Nat Protoc. 2019;14:1772–802.
Bhingardive V, Edri A, Kossover A, Saux GL, Khand B, Radinsky O, Iraqi M, Porgador A, Schvartzman M. Nanowire Based Mechanostimulating platform for tunable activation of natural killer cells. Adv Funct Mater. 2021;31:2103063.
Rostgaard KR, Frederiksen RS, Liu Y-CC, Berthing T, Madsen MH, Holm J, Nygård J, Martinez KL. Vertical nanowire arrays as a versatile platform for protein detection and analysis. Nanoscale. 2013;5:10226–35.
Elnathan R, Holle AW, Young J, George MA, Heifler O, Goychuk A, Frey E, Kemkemer R, Spatz JP, Kosloff A, Patolsky F, Voelcker NH. Optically transparent vertical silicon nanowire arrays for live-cell imaging. J Nanobiotechnol. 2021;19:51.
Brodoceanu D, Alhmoud HZ, Elnathan R, Delalat B, Voelcker NH, Kraus T. Fabrication of silicon nanowire arrays by near-field laser ablation and metal-assisted chemical etching. Nanotechnology. 2016;27:075301.
Elnathan R, Isa L, Brodoceanu D, Nelson A, Harding FJ, Delalat B, Kraus T, Voelcker NH. Versatile particle-based Route to engineer vertically aligned Silicon Nanowire arrays and Nanoscale pores. ACS Appl Mater Interfaces. 2015;7:23717–24.
Rey BM, Elnathan R, Ditcovski R, Geisel K, Zanini M, Fernandez-Rodriguez M-A, Naik VV, Frutiger A, Richtering W, Ellenbogen T, Voelcker NH, Isa L. Fully tunable Silicon Nanowire arrays fabricated by soft nanoparticle templating. Nano Lett. 2016;16:157–63.
He G, Feng J, Zhang A, Zhou L, Wen R, Wu J, Yang C, Yang J, Li C, Chen D, Wang J, Hu N, Xie X. Multifunctional branched nanostraw-electroporation platform for intracellular regulation and monitoring of circulating Tumor cells. Nano Lett. 2019;19:7201–9.
Xie X, Xu AM, Leal-Ortiz S, Cao Y, Garner CC, Melosh NA. Nanostraw-electroporation system for highly efficient intracellular delivery and transfection. ACS Nano. 2013;7:4351–8.
Caprettini V, Cerea A, Melle G, Lovato L, Capozza R, Huang J-A, Tantussi F, Dipalo M, De Angelis F. Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes. Sci Rep. 2017;7:1–8.
Cao Y, Chen H, Qiu R, Hanna M, Ma E, Hjort M, Zhang A, Lewis RS, Wu JC, Melosh NA. Universal intracellular biomolecule delivery with precise dosage control. Sci Adv. 2018;4:eaat8131.
Zhang A, Fang J, Wang J, Xie X, Chen H-J, He G. Interrogation on the Cellular Nano-Interface and Biosafety of repeated Nano-Electroporation by Nanostraw System. Biosensors. 2022;12:522.
Hebisch E, Hjort M, Volpati D, Prinz CN. Nanostraw-assisted Cellular Injection of fluorescent nanodiamonds via direct membrane opening. Small. 2021;17:e2006421.
Zhang A, Yao C, Hang T, Chen M, He G, Chen H-j, Xie X. Spin-Coating-Based fabrication of Nanostraw arrays for Cellular Nano-electroporation. ACS Appl Nano Mater. 2022;5:2057–67.
Messina GC, Dipalo M, La Rocca R, Zilio P, Caprettini V, Proietti Zaccaria R, Toma A, Tantussi F, Berdondini L, De Angelis F. Spatially, temporally, and quantitatively controlled delivery of broad range of molecules into selected cells through plasmonic nanotubes. Adv Mater. 2015;27:7145–9.
Wen R, Zhang AH, Liu D, Feng J, Yang J, Xia D, Wang J, Li C, Zhang T, Hu N, Hang T, He G, Xie X. Intracellular delivery and sensing System based on Electroplated Conductive Nanostraw arrays. ACS Appl Mater Interfaces. 2019;11:43936–48.
Lin ZC, Xie C, Osakada Y, Cui Y, Cui B. Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials. Nat Commun. 2014;5:3206.
Chen Y, Yoh HZ, Shokouhi AR, Murayama T, Suu K, Morikawa Y, Voelcker NH, Elnathan R. Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes. J Nanobiotechnol. 2022;20:406.
Lee K, Lingampalli N, Pisano AP, Murthy N, So H. Physical delivery of macromolecules using high-aspect ratio nanostructured materials. ACS Appl Mater Interfaces. 2015;7:23387–97.
He G, Hu N, Xu AM, Li X, Zhao Y, Xie X. Nanoneedle platforms: the many ways to pierce the cell membrane. Adv Funct Mater. 2020;30:1909890.
Capozza R, Caprettini V, Gonano CA, Bosca A, Moia F, Santoro F, De Angelis F. Cell membrane disruption by vertical micro-/nanopillars: role of membrane bending and traction forces. ACS Appl Mater Interfaces. 2018;10:29107–14.
Saklayen N, Huber M, Madrid M, Nuzzo V, Vulis DI, Shen W, Nelson J, McClelland AA, Heisterkamp A, Mazur E. Intracellular delivery using nanosecond-laser excitation of large-area plasmonic substrates. ACS Nano. 2017;11:3671–80.
Wang Y, Yang Y, Yan L, Kwok SY, Li W, Wang Z, Zhu X, Zhu G, Zhang W, Chen X, Shi P. Poking cells for efficient vector-free intracellular delivery. Nat Commun. 2014;5:4466.
Dixit HG, Starr R, Dundon ML, Pairs PI, Yang X, Zhang Y, Nampe D, Ballas CB, Tsutsui H, Forman SJ, Brown CE, Rao MP. Massively-parallelized, deterministic mechanoporation for intracellular delivery. Nano Lett. 2020;20:860–7.
Tay A, Melosh N. Transfection with nanostructure electro-injection is minimally perturbative. Adv Ther. 2019;2:1900133.
He G, Chen HJ, Liu D, Feng Y, Yang C, Hang T, Wu J, Cao Y, Xie X. Fabrication of various structures of nanostraw arrays and their applications in gene delivery. ACS Appl Mater Interfaces. 2018;5:1701535.
Riaz K, Leung S-F, Fan Z, Lee Y-K. Electric field enhanced 3D scalable low-voltage nano-spike electroporation system. Sens Actuator A Phys. 2017;255:10–20.
Dong Z, Chang L. Recent electroporation-based systems for intracellular molecule delivery. Nanotechnol Precis Eng. 2021;4:045001.
Fajrial AK, Ding X. Advanced nanostructures for cell membrane poration. Nanotechnology. 2019;30:264002.
Shalek AK, Gaublomme JT, Wang L, Yosef N, Chevrier N, Andersen MS, Robinson JT, Pochet N, Neuberg D, Gertner RS, Amit I, Brown JR, Hacohen N, Regev A, Wu CJ, Park H. Nanowire-mediated delivery enables functional interrogation of primary immune cells: application to the analysis of chronic lymphocytic leukemia. Nano Lett. 2012;12:6498–504.
Elnathan R, Delalat B, Brodoceanu D, Alhmoud H, Harding FJ, Buehler K, Nelson A, Isa L, Kraus T, Voelcker NH. Maximizing transfection efficiency of vertically aligned silicon nanowire arrays. Adv Funct Mater. 2015;25:7215–25.
Choi M, Lee SH, Kim WB, Gujrati V, Kim D, Lee J, Kim JI, Kim H, Saw PE, Jon S. Intracellular delivery of bioactive cargos to hard-to‐transfect cells using Carbon Nanosyringe arrays under an Applied Centrifugal g‐Force. Adv Healthc Mater. 2016;5:101–7.
Yarmush ML, Golberg A, Sersa G, Kotnik T, Miklavcic D. Electroporation-based technologies for medicine: principles, applications, and challenges. Annu Rev Biomed Eng. 2014;16:295–320.
Yang Z, Chang L, Chiang C-L, Lee LJ. Micro-/nano-electroporation for active gene delivery. Curr Pharm Des. 2015;21:6081–8.
Henslee BE, Morss A, Hu X, Lafyatis GP, Lee LJ. Electroporation dependence on cell size: optical tweezers study. Anal Chem. 2011;83:3998–4003.
Valley JK, Neale S, Hsu HY, Ohta AT, Jamshidi A, Wu MC. Parallel single-cell light-induced electroporation and dielectrophoretic manipulation. Lab Chip. 2009;9:1714–20.
Guo X, Zhu R. Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array. Sci Rep. 2016;6:1–8.
Lee M, Chea K, Pyda R, Chua M, Dominguez I. Comparative analysis of non-viral transfection methods in mouse embryonic fibroblast cells. J Biomol Tech. 2017;28:67.
Fouriki A, Dobson J. Nanomagnetic gene transfection for non-viral gene delivery in NIH 3T3 mouse embryonic fibroblasts. Materials. 2013;6:255–64.
Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y. Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process. PLoS ONE. 2011;6:e17492.
Slastnikova TA, Ulasov AV, Rosenkranz AA, Sobolev AS. Targeted intracellular delivery of antibodies: the state of the art. Front Pharmacol. 2018;9:1208.
Singh K, Ejaz W, Dutta K, Thayumanavan S. Antibody delivery for intracellular targets: Emergent Therapeutic potential. Bioconjug Chem. 2019;30:1028–41.
Gaston J, Maestrali N, Lalle G, Gagnaire M, Masiero A, Dumas B, Dabdoubi T, Radošević K, Berne P-F. Intracellular delivery of therapeutic antibodies into specific cells using antibody-peptide fusions. Sci Rep. 2019;9:1–12.
Aslanoglou S, Chen Y, Oorschot V, Trifunovic Z, Hanssen E, Suu K, Voelcker NH, Elnathan R. Efficient transmission electron microscopy characterization of cell–nanostructure interfacial interactions. J Am Chem Soc. 2020;142:15649–53.
Weaver JC, Smith KC, Esser AT, Son RS, Gowrishankar T. A brief overview of electroporation pulse strength–duration space: a region where additional intracellular effects are expected. Bioelectrochemistry. 2012;87:236–43.
Gabriel B, Teissie J. Generation of reactive-oxygen species induced by electropermeabilization of chinese hamster ovary cells and their consequence on cell viability. Eur J Biochem. 1994;223:25–33.
Kim TK, Eberwine JH. Mammalian cell transfection: the present and the future. Anal Bioanal Chem. 2010;397:3173–8.
Kitajiri S-i, Sakamoto T, Belyantseva IA, Goodyear RJ, Stepanyan R, Fujiwara I, Bird JE, Riazuddin S, Riazuddin S, Ahmed ZM. Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing. Cell. 2010;141:786–98.
Zaharija B, Samardžija B, Bradshaw NJ. The TRIOBP isoforms and their distinct roles in actin stabilization, deafness, Mental Illness, and Cancer. Molecules. 2020;25:4967.
Bao J, Wang S, Gunther LK, Kitajiri S-i, Li C, Sakamoto T. The actin-bundling protein TRIOBP-4 and-5 promotes the motility of pancreatic cancer cells. Cancer Lett. 2015;356:367–73.
Yoh HZ, Chen Y, Shokouhi A-R, Thissen H, Voelcker NH, Elnathan R. The influence of dysfunctional actin on polystyrene-nanotube-mediated mRNA nanoinjection into mammalian cells. Nanoscale. 2023;15:7737–44.
Carthew J, Abdelmaksoud HH, Cowley KJ, Hodgson-Garms M, Elnathan R, Spatz JP, Brugger J, Thissen H, Simpson KJ, Voelcker NH, Frith JE, Cadarso VJ. Next Generation Cell Culture Tools featuring Micro- and nanotopographies for Biological Screening. Adv Funct Mater. 2022;32:2100881.
Carthew J, Abdelmaksoud HH, Hodgson-Garms M, Aslanoglou S, Ghavamian S, Elnathan R, Spatz JP, Brugger J, Thissen H, Voelcker NH, Cadarso VJ, Frith JE. Precision Surface Microtopography regulates cell fate via changes to Actomyosin Contractility and Nuclear Architecture. Adv Sci. 2021;8:2003186.